Please use this identifier to cite or link to this item: https://app.uff.br/riuff/handle/1/11466
Title: Efeito do consumo da semente de linhaça em ratas castradas: indicadores hormonais e imuno-histoquímicos
Authors: Guerra, Thaís de Rezende Bessa
metadata.dc.contributor.advisor: Coca Velarde, Luis Guillermo
metadata.dc.contributor.advisorco: Boaventura, Gilson Teles
metadata.dc.contributor.members: Cardoso, Gilberto Perez
França, Bartolomeu Expedito da Câmara
Ferreira, Patricia Silva
Issue Date: 2011
Citation: GUERRA, Thaís de Rezende Bessa. Efeito do consumo da semente de linhaça em ratas castradas: indicadores hormonais e imuno-histoquímicos. 2011. 70 f. Dissertação (Mestrado de Ciências Médicas) - Universidade Federal Fluminense, Niterói, 2011.
Abstract: A semente de linhaca tem despertado o interesse da comunidade cientifica por apresentar inumeros beneficios a saude. Objetivou-se analisar a influencia da semente de linhaca em ratas castradas nos indicadores hormonais e nos receptores de estrogeno presentes nos tecidos do utero e figado. Foram utilizadas 21 ratas Wistar e submetidas a ooforectomia bilateral para inducao da menopausa e apos sorteadas aleatoriamente em tres grupos: Grupo Controle (GC) racao de caseina, Grupo Linhaca (GL) racao de caseina, adicionada 25% de semente de linhaca e Grupo Controle Modificado (GCM) racao de caseina, adicionada de fibras e lipidios. Todas as racoes foram elaboradas segundo as recomendacoes da AIN 93 e os animais foram cuidados seguindo os preceitos eticos. Observou-se a cada dois dias o peso(g) e o consumo alimentar(g) dos animais. As ratas foram anestesiadas com ThiopentaxR, realizou-se a coleta do sangue por puncao cardiaca, para determinacao dos niveis hormonais (17ƒÀ.Estradiol, progesterona, FSH, LH e PTH) por radioimunoensaios. Para determinacao dos receptores de estrogeno, os tecidos do utero e figado foram coletados, pesados e armazenados em criogenia. Apos foram processados em blocos parafinados e realizada analise por imunohistoquimica. Para realizar a quantificacao do secoisolaciresional diglicosidio(SDG), este foi isolado da semente de linhaca e utilizada a tecnica de cromatografia liquida de alta resolucao (HPLC). Foi utilizado para analise estatistica o teste nao parametrico de Kruskal-Wallis e aplicado teste post hoc U de Mann-Whitney para comparacao dois a dois atraves do Software S-Plus versao 8.0, com o nivel de p< 0.05, para as diferencas significativas. Ao final dos 180 dias de experimento os resultados observados sugerem que o SDG esta presente na semente de linhaca em 13,5 } 0,4 mg/g e que o consumo de racao no GL(1641,1 }0,9mg/g), GC(1478,4 }0,19mg/g), GCM(1605,8 }1,89mg/g) nao apresentaram diferencas significantes entre os grupos, ja a massa relativa corporal dos animais observamos que o GL(275,5 }33,99mg/g), GC(294,0 }33,19mg/g) e GCM (339,5 }24,69mg/g) sugerem diferencas estatisticas entre os grupos (p< 0,002). Os niveis sericos de 17ƒÀ-estradiol apresentaram valores superiores no GL(48,7 } 12,31 pg/dl), GC(29,0 } 5,4 pg/dl), GCM(36,0 } 5,60 pg/dl), com diferencas estatisticas (p=0,0012) e os niveis sericos de paratohormonio apresentou valor superior no GL(107,5 }5,50 pg/dl), GCM(81,05 }2,88 pg/dl) e GC(2,8 }0,21pg/dl), sugerem diferencas significantes entre os grupos (valor p=0,0001), as concentracoes sericas de LH nos grupos GC (0,56 } 0,12 pg/dl), GCM(0,32 }0,04 pg/dl) e GL (0.13 }0.05pg/dl) apresentaram diferencas estatisticas nos grupos GC e GL (valor p= 0,0168). Ja os niveis sericos de FSH no GL(0,1 } 0,01pg/dl), GC(0,2 }0,002 pg/dl) e GCM(0,1 }0,01pg/dl), sugere diferenca estatistica no GC (p= 0,2431), quanto a concentracao serica de progesterona no GL(1,59 } 0,23), GC (7,62 } 1,0l) e GCM (7,06 } 1,01pg/dl) apresentou diferenca significativa entre os grupos (valor p.0,0001). As analises por imunohistoquimica dos receptores de estrogeno apresentaram-se negativos nos tecidos do utero e figado nos grupos GL, GC e GCM. Considera-se que, o secoisolaciresional diglicosidio presente na semente de linhaca a 25% influenciou no consumo alimentar, na massa relativa corporal dos animais e nos niveis de estradiol e paratohormonio e nao foi suficiente para causar modificacao nos niveis hormonais de LH, FSH e progesterona e nos receptores hormonais dos tecidos do utero e figado das ratas ooforectomizadas
metadata.dc.description.abstractother: The flaxseed has attracted the interest of the scientific community by presenting numerous health benefits. The objective was to analyze the influence of flaxseed in rats castrated on hormone levels and estrogen receptors in the tissues of the uterus and liver. We used 21 Wistar rats bilateral oophorectomy and to induce menopause and after randomly divided three groups: control group (CG) ration of casein, Flaxseed Group (GF) diet of casein, 25% of added flaxseed and Control Group modified (CCM) casein diet, with added fiber and lipids. All diets were prepared according to the recommendations of the AIN 93 and ethics animals. There was every other day weight (g) and food intake (g) of animals. The rats were anesthetized with Thiopentax ®, there was blood collection by cardiac puncture for determination of hormone levels (17β-estradiol, progesterone, FSH, LH and PTH) by radioimmunoassay. For determination of the estrogen receptors of the uterus and liver tissues were collected, weighed and stored in cryogenic after were processed in paraffin blocks and performed immunohistochemical analysis. To perform the quantification of secoisolaciresional diglicosídio, this was isolated from flaxseed and used the technique of high performance liquid chromatography (HPLC). Was used for statistical analysis the nonparametric Kruskal-Wallis test and applied post hoc Mann-Whitney U for comparison two by two through the S-Plus software version 8.0, with the level of p <0.05 for significant differences. At 180 days of experiment the observed results suggest that SDG is present in flaxseed at 13.5 ± 0.4 mg/g and the feed intake in FG (1641.1 ± 0.9), GC ( 1478.4 ± 0.1) and GCM (1605.8 ± 1.8) showed no significant difference between the groups, since the animals body mass relative observed that the FG (275.5 ± 33.9), GC ( 294.0 ± 33.1) and GCM (339.5 ± 24.6), suggesting that there was statistical difference between the groups GL and GC (P <0.0013), because the GCM was no significant difference with other groups (p <0.002). Although 17β-estradiol have higher value from the GL (48.7 ± 12.31 pg/dl), GC (29.0 ± 5.4 pg/dl), GCM (36.0 ± 5.60 pg/dl), statistical difference (p= 0.0012) and PTH in FG (107.5 ± 5.50pg/dl), GCM (81.05 ± 2.88 pg/dl) and GC (2.8 ± 0.21 pg/dl ), suggesting a significant difference between groups (p=0.0001), serum concentrations of LH in CG (0.56 ± 0.12 pg/dl), GCM (0.32 ± 0.04 pg/dl) and FG (0.13 ± 0.05pg/dl) showed a statistical difference in CG and FG (p=0.0168), as serum levels of FSH on GL (0.1 ± 0.01pg/dl), the CG (0.2 ± 0002 pg/dl) and the GCM (0.1 ± 0.01pg/dl) suggest a statistical difference in CG (p=0.2431), and the serum concentration of progesterone in GL (1.59 ± 0.23), GC (7.62 ± 1.0L) and GCM (7.06 ± 1.01pg/dl) significant difference between groups (p≤0.0001). Analyzing the estrogen receptors in the uterus and liver tissues revealed negativity. We believe that secoisolaciresional diglicosídio present in flaxseed to 25% influence on food intake relative to body mass in animals and that despite its influence on levels of estradiol and PTH was not enough to cause changes in hormone levels LH, FSH and progesterone, and presented to the estrogen receptor antagonist in tissues of the uterus and liver of ovariectomized rats
URI: https://app.uff.br/riuff/handle/1/11466
Appears in Collections:PPGCM - Teses e Dissertações

Files in This Item:
File Description SizeFormat 
THAIS DE REZENDE BESSA GUERRA DISSERTAÇAO.pdf754.02 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons