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Title: O efeito da talidomida sobre a carcinogênese cutânea experimental em camundongos BALB/c
Other Titles: The effect of thalidomide on angiogenesis on experimental mouse skin carcinogenesis
Keywords: Neoplasia;  Pele;  Carcinogênese química experimental;  Camundongo BALB/c;  Talidomida;  Angiogênese;  Neoplasia;  Skin;  Experimental chemical carcinogenesis;  BALB/c mouse;  Thalidomide;  Angiogenesis;  MEDICINA;  PATOLOGIA INVESTIGATIVA;  Carcinógenos;  Anticarcinógenos;  Neoplasias cutâneas
Issue Date: 17-Feb-2009
Abstract: The experimental model of mouse skin carcinogenesis has been largely used for investigating cancer pathogenesis and therapy. In this work it is used this classical model in order to assess the antineoplastic potential of thalidomide in BALB/c mice. Animals were distributed into four experimental groups: 1) dimetilbenzantracene (DMBA), carcinogenic agent (diluted into acetone), 2) DMBA + thalidomide (diluted into dimetil sulfoximide - DMSO), 3) DMBA + DMSO and 4) Acetone. Animals were also divided into two experimental groups, in experiment 1, the mice received thalidomide at the same time of the carcinogenesis induction and in experiment 2, mice received thalidomide after the tumoral lesions appearance. Body weight was measured once a week. Animals were killed after 14 (experiment 1) and 22 weeks (experiment 2). Skin tumors were removed, and histopathological and immunohistochemical analyses were accomplished. Immunohistochemistry was used for the assessment of angiogenesis, which was measured using the antibody antifactor VIII (endothelial antigen indicative of vascular proliferation). All the animals developed multiple skin tumors, which varied from papillomas to squamous cells carcinomas, including the thalidomide treated group. In experiment 1, the thalidomide inhibited the total number of tumors per mouse, besides inducing a decrease of malignancy of lesions. In experiment 2 all the animals had malignant lesions. Thalidomide was unable to inhibit tumoral angiogenesis and weight loss in both experiments. Taken together theses results, it can be concluded that thalidomide has a positive role in the prevention of malignization of mouse skin tumors; however this effect is not due to angiogenesis inhibition. Besides, it seems to be useless when administrated to mice bearing established malignant tumoral lesions
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