COMPARAÇÃO ENTRE ISOLAMENTO BACTERIOLÓGICO CONVENCIONAL E PCR NA DETECÇÃO DE SALMONELLA SPP. EM AMOSTRAS DE CARNE DE FRANGO ARTIFICIALMENTE CONTAMINADAS E DE CAMPO
Método convencional
PCR
Salmonella spp Contaminação da carne
Carne de ave
Reação em cadeia da polimerase
Produção intelectual
Comparison
Conventional culture-based method
PCR, Salmonella spp
Contamination of meat, poultry, Polymerase chain reaction
Intellectual Production
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
Abstract
Salmonellosis is one of the most challenging zoonoses to public health, due to its
difficult control, high morbidity, as well as its economic and social impacts. Most
cases of Salmonella food borne diseases are caused by the ingestion of
contaminated food, especially poultry meat and poultry products processed under
improper conditions. Conventional culture-based methods are widely used to detect
Salmonella spp. in foods and it is contemplated in Brazilian law, although the
technique is laborious and time-consuming. Polymerase Chain Reaction (PCR)
which is a fast, sensitive and useful method to detect food borne pathogens, such as
Salmonella spp., has been quite researched and gradually employed in food
industries. The objective of this study is to compare conventional culture-based
method to PCR for Salmonella spp. detection in poultry meat. Thirty-four artificially
inoculated chicken meat with S. Enteritidis samples and thirty-one naturally
contaminated samples have been analyzed. Both methods were capable of detecting
Salmonella spp. in artificially contaminated samples. PCR has been faster on
detection of Salmonella spp. Only conventional culture-based methods could detect
Salmonella spp. in naturally contaminated samples.
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Document type
DissertaçãoFormat
application/pdf
Subject(s)
ComparaçãoMétodo convencional
PCR
Salmonella spp Contaminação da carne
Carne de ave
Reação em cadeia da polimerase
Produção intelectual
Comparison
Conventional culture-based method
PCR, Salmonella spp
Contamination of meat, poultry, Polymerase chain reaction
Intellectual Production
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA