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Title: Caracterização físico-química e avaliação in vitro de hidroxiapatitas substituídas com 5% e 1% de metais
Keywords: Avaliação in vitro;  Apatitas 3;  Cultura de células;  Metais;  Normas técnicas;  Biomateriais;  Citotoxicidade;  Apatitas;  Testes in vitro;  Biomaterials;  Cytotoxicity;  Apatite;  In vitro tests
Issue Date: 17-Dec-2009
Abstract: This work intended to evaluate the physical-chemical properties and in vitro of apatites modified by the incorporation of different metals. They were produced by the wet via and powders not sinterized of hydroxyapatite (HA) and apatite containing 5% of Pb, Sr, V, Co, Zn, Fe, Cu, Mg and spheres of HA and apatite with 1% of Pb, Sr, Co, Zn, Fe, Cu, Mg, were characterized by X Rays diffraction, Fourier Transformed Infrare Fluorescence, atomic absorption, ICP, dissolution in DMEM and scanning electron microscopy (SEM). Extracts of the HA and of the apatites modified with 1 and 5% of metal were obtained according to the ISO 10993 5-12. To cytotoxicity of the apatites modified with 5% of metal was determined in function of the cells number, while the cytotoxicity of metal apatites modified with 1% of metal was carried out utilizing a kit for determination of the reduction of the XTT and incorporation of neutral red and crystal violet. Discs (14 x 1mm of thickness) were utilized for evaluate the relation cell-biomaterials and, after 4 and 24 of the osteoblasts seeding the adhered cells were quantified in Neubauer s chamber; discs containing cells were submitted to SEM. The rate of cells in apoptosis was determined by anexine V kit for the spheres of apatite modified with 1% of metal. The apatites produced did not presented other phase and showed itself crystalline. CuHA, CoHA, MgHA and SrHA5% presented evident dissolution in DMEM; CuHA, CoHA, MgHA, ZnHA, PbHA and SrHA1% also showed dissolution in DMEM. The disks of apatite not sinterized showed minor roughness than the sinterized apatite containing alginate. Sr, V, Co and FeHA 5% showed no spreading even after 24h after seeding; onto 1% apatites disks the same was observed after 24 hours. Regarding the mechanism of cellular death by apoptose was possible to evaluate that CuHA1% and ZnHA1% presented minor percentage of apoptotic cells in opposition to the Co and MgHA1%. In conclusion it was possible to obtain the crystalline and free of other phases metal modified apatites up to 1 and 5%, but the real concentration of the present metals does not correspond upon theoretically proposing. The substitution of the apatites by metals modifies its toxicity in vitro
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