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Other Titles: Proteomic aspects in sponge Haliclona SP Submitted to cadmiun Stress for environmental Studies
Keywords: Biomonitoramento;  Biomarcadores;  Proteínas;  Esponja Marinha;  Cádmio;  Biomonitoring;  Biomarkers;  Proteins;  Marine Sponge;  Cadmium
Issue Date: 28-May-2012
Abstract: In this work we used the cultivated marine sponge Haliclona sp. as a biological matrix to evaluate molecular responses caused by Cd2+ exposure in different times. The separation by 1D-PAGE using sponge samples labeling with monobromobimane and 2D-PAGE allied with the mass spectrometry (ESI-QTOF-MS) were used to identify differentially expressed proteins between control and exposure conditions. Furthermore, histological techniques were used to show morphological effects caused by exposure to this metal. The largest accumulation of Cd2+ was observed at the 326 hours. In addition, histology and 1D-PAGE results showed also a greater cell damage and induction of a low-molecular-mass and SH-rich protein in this exposure time. With the use of 2D-PAGE was possible to detect differentially expressed in 46 spots proteins in the 326 hours samples. The use of two distinct steps of protein extraction showed differences in number and intensity of spots detected. The solvent precipitation step had a greater number of spots while the heat denaturation had a greater intensity. The use of mass spectrometry together blastp bioinformatics tool was able to identify 17 proteins related to the sponge (24%), yeast (29%) and bacteria (47%). Among these identified proteins, these ones could be related to the functions as cell proliferation and differentiation, energy obtaining and, especially, repair to oxidative stress functions. The identification of differentially expressed proteins together by the histological results, was able to indicate the effects in marine sponge caused by exposure to Cd+2, indicating the potential use of these molecular responses in Haliclona sp. as a tool to evaluation of metal contamination in marine coastal environments.
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