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|Title:||Detecção imunohistoquímica de HSP90 e KI-67 em pterígio|
|Keywords:||Imunohistoquímica; Hsp90; Ki-67; Pterígio; Immunohistochemistry; Hsp90; Ki-67; Pterygium|
|Abstract:||Purpose: To examine the immunohistochemical expression of heat shock protein 90 (Hsp90) and Ki-67 protein in human pterygium. Materials and methods: Tissues obtained during pterygium surgery of 15 patients who underwent the bare-sclera procedure and 10 normal conjunctivae were studied. All of these pterygia were primary ones. Recurrent pterygia were excluded. Normal bulbar conjunctivas (2 x 2 mm) were obtained from the nasal region close to the limbus from patients during their cataract and retina surgeries. Immunohistochemical detection of Hsp90 and Ki67 was done using the streptavidin biotin method in paraffin embedded tissue sections. All cases were treated by the same surgeon at the Universitary Hospital Universidade Federal Fluminense. Immunohistochemical study was made at Department of Ophthalmology and Pathology, The McGill University Health Center and Henry C.Witelson Ocular Pathology Laboratory, Montreal, Quebec, Canada. The unpaired Student s t test was used to compare the samples. To determinate the degrees of freedom was used the Aspin-Welch test. A P value of <0,05 was considered statistically significant. Results: The percentage of cells stained for Hsp90 was greater for pterygium epithelium (76 ± 10.8) than for normal conjunctiva (1.4 ± 0.8). In each pterigyum sample more than 60% of cells were positive. The differences in positive cells between normal and pterigyum epithelium were highly significant for Hsp90 (P < 0,001). Pterygium epithelium also showed a higher percentage of cells that stained for Ki-67 (10.1 ± 9.5) than for normal conjunctiva (2.1 ± 1.9). The differences in positive cells were also statistically significant for Ki-67 (P < 0.01). Although there was significant difference in the majority of samples it was observed that in some samples there was no difference between normal and pterygium epithelium for Ki-67. Conclusion: Our results indicate an abnormal expression of Hsp and ki-67 in pterygium samples when compared to normal conjunctiva. The finding of abnormal expression of Hsp90 in pterygium samples can stimulate new research about pterygium and its recurrence|
|Appears in Collections:||TEDE sem arquivo|
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